Fast and real-time electrical transistor assay for quantifying SARS-CoV-2 neutralizing antibodies

Abstract Due to the SARS-CoV-2 pandemic renewed attention has been directed towards viral neutralization assays and neutralizing antibodies quantification, for vaccine pre-clinical trials determining efficacy over time. The gold standard assess antibody titer is plaque reduction test, an end-point assay which evaluates highest serum dilution that neutralizes replication, by inspecting cytopathic effect induced on cell cultures. Here, we use planar, PEDOT:PSS-based organic electrochemical transistors real-time, remote-controlled, reliable fast electrical monitoring of SARS29 CoV-2 Vero E6 lines, allowing quantification titer. Our low-cost scalable device potential speed-up large-scale screening without need cancerous staining or highly specialized operators. Finally, technology could be easily transferred response different viruses in their permissive substrates.